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Tn10 transposon mutagenesis.
A large number of Escherichia coli strains have been constructed by transposon mutagenesis with Tn10, a multicopy plasmid of a class of minicircles. The method is based on a mechanism, by which double-strand breaks (DSBs) produced at random locations within the plasmid are repaired in such a way that the chromosomal region from which the transposon has inserted is

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